Publications

3d Bone Tissue Engineered witoactive Microspheres in Simulated Microgravity

Qing-Qing Qiu, Paul Ducheyne, Portonovo S. Ayyaswamy

In Vitro Cell. Dev. Biol.~Animal 37:157-165, March 2001

calcium phosphate; microgravity; bone formation; shear stress; degradable microspheres; drug delivery.

A Clinically Suitable Ex-Vivo Expansion Culture System for LTC-IC and CFC Using Stroma-Conditioned Medium

R. Bhatia,P.B. McGlave, J.S. Miller, S. Wissink, W. Lin, C.M. Verfaillie

Experimental Hematology. 1997. 25:980-991.

Hematopoietic progenitors; Stem celIs; Ex vivo expansion; Platelet factor-4; Chronic myelogenous leukemia

A clinical-scale selective allodepletion approach for the treatment of HLA-mismatched and matched donor-recipient pairs using expanded T lymphocytes as antigen-presenting cells and a TH9402-based photodepletion technique

Stephan Mielke, Raquel Nunes, Katayoun Rezvani, Vicki S. Fellowes, Annie Venne, Scott R. Solomon, Yong Fan, Emma Gostick, David A. Price, Christian Scotto, Elizabeth J. Read, and A. John Barrett1

Blood. 2008 April 15; 111(8): 4392–4402. 

A Comparative Analysis of Serum and Serum-free Media for Generation of Clinical Grade DCs

Chiara Napoletano, Dora Pinto, Filippo Bellati, Federica Taurino, Hassan Rahimi, Federica Tomao, Pierluigi Benedetti Panici, Aurelia Rughetti, Luigi Frati, Marianna Nuti

J Immunother 2007;30:567-576

dendritic cells, serum-free culture, immunotherapy

A Comparison of the Effectiveness of Lak Cells Grown in FEP Teflon Bags vs. Cells Grown in PL732 Bags

John R. Yannelli

A Comparison of The Expansion and Functional Capabilities of Dendritic Cells Derived from Blood Monocytes vs Mobilized Blood CD34+ Cells, Matured With Either CD40L or TNF-α/IFN-α

B. Chen, P. Stiff, G. Sloan, J. Kash, R. Manjunath, B. Nickoloff

Departments of Medicine and Pathology, Loyola University Medical Center, Maywood, IL

A functional comparison of mature human dendritic cells prepared in fluorinated ethylene-propylene bags or polystyrene flasks

Roger J. Kurlander, Abdul Tawab, Yang Fan, Charles S. Carter, Elizabeth J. Read

Transfusion 2006;46: 1494-1504.

A novel strategy for selective depletion of host-reactive donor lymphocytes by photodepletion is efficient at clinical scale conditions and preserves foxp3+ regulatory T cells

S. Mielke, K. Rezvani, V. Fellowes, A. Venne, S.R. Solomon, Y. Fan, N. Hensel, C.S. Carter, E.J. Read, A.J. Barrett

Biology of Blood and Marrow Transplantation
Volume 12, Issue 2, Supplement 1, February 2006, Page 103 

A Protocol for Generation of Clinical Grade mRNATransfected Monocyte-Derived Dendritic Cells for Cancer Vaccines

L .J. Mu, G. Gaudernack, 

Blackwell Publishing Ltd. Scandinavian Journal of Immunology 58, 578-586

A Rapid Seeding Technique for the Assembly of Large Cell/ Scaffold Composite Constructs

Luis A. Solchaga, 

Tissue Eng. 2006 July ; 12(7): 1851–1863.

A Role for a RhoA/ROCK/LIM-Kinase Pathway in the Regulation of Cytotoxic Lymphocytes

Zhenkun Lou, Daniel D. Billadeau, Doris N. Savoy, Renee A. Schoon, Paul J. Leibson

The Journal of Immunology, 2001, 167: 5749-5757.  

Adherent-Free Generation of Functional Dendritic Cells From Purified Blood Monocytes in View of Potential Clinical Use

J. Bernard, D. Ittelet, A. Christoph, G. Potron, J.C. Adjizian, S.Kochman, M. Lopez

Hematology and Cell Therapy (1998) 40:17-26.

An Improved Method for the Generation of Human Lymphokine Activated Killer Cells.

Susan G. Dickerson, Ann Mrowca, Robert K. Oldham, Eileen Sharp, Gary B. Thurman, John R. Yannelli

Journal of Immunological Methods. 1987

Analytical model for residual stresses in polymeric containers during cryogenic storage of hematopoietic stem cells 

David M. Saylor, 

Acta Biomaterialia 

Blood bags; Hematopoietic stem cells (HSC); Cryogenic storage; Failure model; Residual stresses; Thermal expansion mismatch; Gas evolution

Catastrophic failures of freezing bags for cellular therapy products: description, cause, and consequences

H.M. Khuu, H. Cowley, V. David-Ocampo, C.S. Carter, C. Kasten-Sportes, A.S. Wayne, S.R. Solomon, M.R. Bishop, R.M. Childs, E.J. Read

Cytotherapy (2002) Vol. 4, No. 6, 539–549

cryopreservation, cellular therapy product, aseptic methods, plastic bags, fluorinated ethylene propylene (FEP),
bacterial contamination

Cd34+ Peripheral Blood Progenitor Cell (Pbpc) Derived Dendritic Cells (Dc): Optimal Culture Conditions

R. Yerushalmi, 

ISHAGE San Diego 2000 Abstrct - abstract # 106

Dendritic cell expansion

Change in organic carbon in forest plantation soils in eastern Australia

John Turner, Marcia Lambert

Forest Ecology and Management, Volume 133, Issue 3, 15 August 2000, Pages 231-247

Characterization of biochemical changes occurring during storage of red cells

G. Moroff, D. Dende

Transfusion 1983;23:484-469.

R.K. Oldham, P. Miller

Biological Therapeutics Branch, Biological Response Modifiers Program, National Cancer Institute, Frederick Cancer Research Facility and the Viral Carcinogenesis Program, Basic Research Program, Frederick Cancer Research Facility, Frederick, MD. Journal of Immunological Methods. 1984. 70:245-255.

Chelatable cellular copper modulates differentiation and self-renewal of cord blood–derived hematopoietic progenitor cells 

Toni Peled, Elina Glukhman, Nira Hasson, Sophie Adi, Harel Assor, Dima Yudin, Chana Landor, Julie Mandel, Efrat Landau, Eugenia Prus, Arnon Nagler, Eitan Fibach

Experimental Hematology, Volume 33, Issue 10, October 2005, Pages 1092-1100 

Chemically defined medium and Caenorhabditis elegans

Nathaniel J. Szewczyk, Elena Kozak, Catharine A. Conley

BMC Biotechnology 2003, 3:19

Clinical Experience with Ex Vivo Expansion of Haematopoietic Progenitor Cells.

Josy Reiffers, C. Cailliot, B. Dazey, I. McNiece, P. Duchez, A. Pigneux, J. Caraux, T. Cousin, B. Bussiere, J.M. Boiron

Clinically Relevant Expansion of Hematopoietic Stem Cells with Conserved Function in a Single-Use, Closed-System Bioprocess 

Gerard J. Madlambayan, Ian Rogers, Kelly A. Purpura, Caryn Ito, Mei Yu, Daniel Kirouac, Robert F. Casper, Peter W. Zandstra

Biology of Blood and Marrow Transplantation
Volume 12, Issue 10, October 2006, Pages 1020-1030 

Stem cell expansion; Bioprocess development; Hematopoiesis; Cord blood, Cell Sample Collection and Purification
Bioprocess Assembly
Cell Seeding and Culture
Subpopulation Selection and Media Dilution/Exchange in the Bioprocess
Phenotypic Analysis
CFC and LTC-IC Assays
Lymphopoietic Assays
Transplantation of Cells into NOD/SCID Mice
Statistical Analysis
Results
Closed-System Bioprocess Development
Robust Total and Progenitor Cell Expansion in the Closed-System Bioprocess
Human Cell Engraftment in NOD/SCID Mice
Identification of Lymphoid Progenitors in Bioprocess-Cultured Cells

Closing the manufacturing process of dendritic cell vaccines transduced with adenovirus vectors

Dumrul Gulen, Fuminori Abe, Sarah Maas, Elizabeth Reed, Kenneth Cowan, Samuel Pirruccello, James Wisecarver, Phyllis Warkentin, Matt Northam, Orhan Turken, Ugur Coskun, Joe Senesac, James E. Talmadge

International Immunopharmacology, Volume 8, Issues 13-14, 20 December 2008, Pages 1728-1736

Dendritic-cells, Elutriation, Closed system, DC vaccine

Commercially Available Gas-Permeable Cell Culture Bags May Not Prevent Anoxia in Cultured or Shipped Islets 

E.S. Avgoustiniatosa, B.J. Heringa, P.R. Rozaka, J.R. Wilsonb, L.A. Tempelmanc, A.N. Balamurugana, D.P. Welch, B.P. Weegmana, T.M. Suszynskia, K.K. Papasa

Transplantation Proceedings
Volume 40, Issue 2, March 2008, Pages 395-400 

Comparison of genotoxic damage in monolayer cell cultures and three-dimensional tissue-like cell assemblies

E. Behravesh, K. Emami, H. Wu, S. Gonda

Advances in Space Research, Volume 35, Issue 2, 2005, Pages 260-267

Comprehensive analysis of citrate effects during plateletpheresis in normal donors

Charles D. Bolan, Sarah E. Greer, Stacey A. Cecco, Jaime M. Obluas, Nadja N. Rehak, Susan F. Leitman

Transfusion 2001;41:1165-1171.

Conservation of Hematopoietic Progenitor Cells for 7 Days in a DMSO-Free, Closed and Automated System.

T. Jonuleit, H. Glockner, H.D. Lemke

Acordis Research Obernburg. Germany (ND)

DMSO-free, conservation, closed
system, stem cell.

Controlled study of citrate effects and response to IV calcium administration during allogeneic peripheral blood progenitor cell donation

Charles D. Bolan, Stacey A. Cecco, Robert A. Wesley, McDonald Horne, Yu Ying Yau, Alan T. Remaley, Richard W. Childs, A. John Barrett, Nadja N. Rehak, Susan F. Leitman

Transfusion 2002;42:935-946.

Cord Blood Banking: Volume Reduction Using "Procord" Terumo Filter

L. Dalcortivo, J. Robert, C. Mangin, T. Sameshima, S. Kora, E. Gluckman M. Benbunan, J.P. Marolleau

Journal of Hematotherapy & Stem Cell Research 9: 885-890 (2000)

Cord blood banking, cryopreservation, CD34

Cryopreservation of Hematopoietic Stem Cells

David Berz, Elise M. McCormack, Eric S. Winer, Gerald A. Colvin, Peter J. Quesenberry

Am J Hematol. 2007 June ; 82(6): 463–472.

Cryopreservation of Hematopoietic Stem Cells_2

Patrick J. Stiff

Lasky L and Warkentin P,eds. Marrow and Stem Cell Processing for Transplantation Bethesda. MD: American Association of Blood Banks, 1995

Culture conditions affect the ability of ex vivo expanded peripheral blood progenitor cells to accelerate hematopoietic recovery 

Ronald L. Paquette, Sanaa T. Dergham, Ellen Karpf, He-Jing Wang, Dennis J. Slamon, Larry Souza, John A. Glaspy

Experimental Hematology
Volume 30, Issue 4, April 2002, Pages 374-380 

Culture Medium and Protein Supplementation in the Generation and Maturation of Dendritic Cells

P.J. Royer, S. Tanguy-Royer, F. Ebstein, C. Sapede, T. Simon, I. Barbieux, R. Oger, M. Gregoire

Scandinavian Journal of Immunology, Volume 63 Issue 6 Page 401-409, June 2006

Culture of Human Tumor Infiltrating Lymphocytes in Hollow Fiber Bioreactors

Richard A. Knazek, Yan-Wan Wu, Paul M. Aebersold, Steven A. Rosenberg

Hollow fiber; Tumor infiltrating lymphocyte; Interleukin-2; Cell culture; Lymphocyte

Dendritic Cell Subsets and Lineages, and Their Functions in Innate and Adaptive Immunity

Yong-Jun Liu

Cell, Vol. 106, 259-262, August 10, 2001

Dendritic Cells as Vectors for Therapy

Jacques Banchereau, Beatrice Schuler-Thumer, A. Karolina Palucka, Gerold Schuler

Cell, Vol. 106, 271-274, August 10, 2001

Dendritic Cells: Specialized and Regulated Antigen Processing Machines

Ira Mellman, Ralph M. Steinman

Cell, Vol. 106, 255-258, August 10, 2001

Development of an Automated Closed System for Generation of Human Lymphokine-Activated Killer (LAK) Cells for use in Adoptive Immunotherapy.

L.M. Muul, K. Nason-Burchenal, C.S. Carter, H. Culls, D. Slavin, C. Hyatt, E.P. Director, S.F. Leitman, H.G. Klein, Steven A. Rosenberg. 

Journal of Immunological Methods, 101. 1987. 171-181.

Lymphokine·activated klller cell; Leukapheresis; Adoptive immunotherapy: Interleukin-2; recombinant; cell culture; closed system

Differential Kinetics of Primitive Hematopoietic Cells Assayed In Vitro and In Vivo During Serum-Free Suspension Culture of CD34+ Blood Progenitor Cells.

Deiter Mobest, S.R. Goan, I. Junghahn, J. Winkler, I. Fichtner, M. Hermann, M. Becker, E. De Lima-Hahn, R. Henschler

Stem Cells 1999; 17:152-161.

Hematopoietic progenitor cells· Serum-free medium· Trauspiantation- Ex rivo expansion

Disposable Equipment: A Mainstay in Bioprocessing

Richard Greene, Rita D'Aquino

www.cepmagazine.org November 2002 CEP

Effective Ultraviolet Irradiation of Platelet Concentrations in Teflon Bags.

S.M. Capon, R.A. Sacher, H.J. Deeg 

Effects of Adherence, Activation and Distinct Serum Proteins on the In Vitro Human Monocyte Maturation Process.

Y. Akiyama, R. Griffith, P. Miller, G.W. Stevenson, Stacy Lund, D.J. Kanapa, H.C. Stevenson

Clinical Investigations Section, Biological Therapeutics Branch, Biological Response Modifiers Program, Division of Cancer Treatment, National Cancer Institute, Frederick Cancer Research Facility, Frederick, MD. Journal of Leukocyte Biology. 1988. 43:224-231.

elutriation, monocyte maturation, serum·free media

Efficient Serum-Free Retroviral Gene Transfer into Primitive Human Hematopoietic Progenitor Cells by a Defined, High-Titer, Nonconcentrated Vector-Containing Medium.

Hanno Climm, K. Flugge, D. Mobest, V. M. Hofmann, J. Postmus, R. Henschler, W. Lange, J. Finke, H-P. Kiem, G. Schulz, F. Rosenthal, R. Mertelsmann, C. Von Kalle. 

Human Gene Therapy 9:771-778 (April 10, 1998).

T. Lightfoot, J. Gallelli, K. Matsuo, S.W. Kwon, S.F. Leitman, D.F. Stroncek

Vox Sanguinis. 80(2):106-111, February 2001.

granulocytes • granulocyte concentrates • granulocyte transfusions • neutrophils

Evidence of similar effects of short-term culture on the initial repopulating activity of mobilized peripheral blood transplants assessed in NOD/SCID-β2microglobulinnull mice and in autografted patients 

Hanno Glimm, Manfred Schmidta, Marlene Fischera, Silke Klingenberga, Winand Langec, Cornelius F. Wallera, Connie J. Eavesd, Christof von Kalle

Experimental Hematology
Volume 33, Issue 1, January 2005, Pages 20-25

Ex vivo expanded peripheral blood progenitor cells provide rapid neutrophil recovery after high-dose chemotherapy in patients with breast cancer 

Ian McNiece, Roy Jones, Scott I. Bearman, Pablo Cagnoni, Yago Nieto, Wilbur Franklin, John Ryder, Andrea Steele, Judy Stoltz, Peggy Russell, Janet McDermitt, Christopher Hogan, James Murphy, Elizabeth J. Shpall 

Blood, 1 November 2000, Vol. 96, No. 9, pp. 3001-3007 

Ex Vivo Expansion and Differentiation of CD34+ Cells Into Red Blood Cell Precursors

A. Caraux, G. Stoney, B. Kern, G. Molineux, R. Briddell

Amgen Ine., Thousands Oaks, CA, U.S.A.

Ex Vivo Expansion and Th1/Tc1 Maturation of Umbilical Cord Blood T Cells by CD3/CD28 Costimulation 

Melissa A. Mazur, Craig C. Davis, Paul Szabolcs

Biology of Blood and Marrow Transplantation
Volume 14, Issue 10, October 2008, Pages 1190-1196 

Ex vivo expansion of cord blood mononuclear cells on mesenchymal stem cells.

I. McNiece, J. Harrington, J. Turney, J. Kellner, E.J. Shpall

Cytotherapy. 2004;6(4):311-7

Ex Vivo Expansion of Hematopoietic Cells

I. McNiece, E. Zaniani, E.J. Shpall

Exp Hematol. 2004 May;32(5):409-10

Ex vivo expansion of megakaryocyte precursors from umbilical cord blood CD34+ cells in a closed liquid culture system

Peter H. Shaw, Devin Gilligan, Xue-Mei Wang, Peter F. Thall, Seth J. Corey

Biology of Blood and Marrow Transplantation
Volume 9, Issue 3, March 2003, Pages 151-156 

Megakaryocyte; Umbilical cord blood; Expansion; Precursor, Mononuclear cell processing

Ex vivo expansion of tumor specific lymphocytes with IL-15 and IL-21 for adoptive immunotherapy in melanoma 

Eduardo Huartea, Jan Fishera, Mary Jo Turkb, Diane Mellingera, Cathy Fostera, Benita Wolfc, Kenneth R. Meehana, Camilo E. Fadula, Marc S. Ernstoff

Cancer Letters
Volume 285, Issue 1, 18 November 2009, Pages 80-88 

T Central Memory; Melanoma; Adoptive immunotherapy; IL-2; IL-15; IL-21

Excellent retroviral transduction efficiency achieved with GMP grade American fluoroseal bags, comparable to tissue culture plates or Nexell X-fold bags 

P.S. Beckera, A.N. Wilksa, B.C. Bearda, S. Chiena, K. Yaraeia, H.-P. Kiema

Blood Cells, Molecules, and Diseases
Volume 40, Issue 2, March-April 2008, Pages 254-255 

Expansion and Activation of Natural Killer Cells for Cancer Immunotherapy

Duck Cho, Dario Campana

Korean J Lab Med 2009;29:89-96
DOI 10.3343/kjlm.2009.29.2.89

NK cells, Cell therapy, Acute myeloid leukemia, Acute lymphoblastic leukemia, Chimeric
receptors

Fibrinogen Activates NF-kB Transcription Factors in Mononuclear Phagocytes. 

R.G. Sitrin, P.M. Pan, S. Srikanth, R.F. Todd III. 

The Journal of Immunology, 1998, 161: 1462-1470.

Future Therapy for Sickle Cell Disease

Elliott Vichinsky, Carlo Brugnara, Lori Styles, John Wagner

American Society of Hemotology

Good manufacturing practices production of natural killer cells for immunotherapy: a six-year single-institution experience

David H. McKenna, Jr., Darin Sumstad, Nancy Bostrom, Diane M. Kadidlo, Susan Fautsch, Sarah McNearney, Rose DeWaard, Philip B. McGlave, Daniel J. Weisdorf, John E. Wagner, Jeffrey McCullough, Jeffrey S. Miller

Transfusion 2007;47:520-528.

Growing Cholesterol-Dependent NSOMyeloma Cell Line in the Wave Bioreactor System: Overcoming Cholesterol-Polymer Interaction by Using Pretreated Polymer or Inert Fluorinated Ethylene Propylene

Judith Kadarusman, Ravi Bhatia, John McLaughlin, WengLong R. Lin

Biotechnol. Prog. 2005, 21, 1341-1346

fluorinated ethylene propylene (FEP) 

Hematopoiesis on Nylon Mesh Templates; Comparative Long-Term Bone Marrow Culture and the Influence of Stromal Support Cells

Brian A. Naughton, Gail K. Naughton

Molecular and Cellular Controls of Hematopoiesis Volume 554 of the Annals of the New York Academy of Sciences May 1, 1989

Stromal; hematopoietic stem cell; long-term bone marrow cultures 

Hepatitis B Transmission From Contaminated Cryopreservation Tank

R. S. Tedder, M. A. Zuckerman, A. H. Goldstone, A. E. Hawkins, A. Fielding, E. M. Briggs, D. Irwin, S. Blair, A.M. Gorman, K.G. Patterson, D.C. Linch, J. Heptonstall, N.S. Brink

Heterogeneity in Macrophage Phagocytosis of Staphylococcus aureus Strains: High-Throughput Scanning Cytometry-Based Analysis

Glen M. DeLoid, Timothy H. Sulahian, Amy Imrich, Lester Kobzik

Identification of TTP mRNA targets in human dendritic cells reveals TTP as a critical regulator of dendritic cell maturation

Jillian Emmons, W.H. Davin Townley-Tilson, Kristen M. Deleault, Stephen J. Skinner, Robert H. Gross, Michael L. Whitfield, Seth A. Brooks

RNA. 2008 May; 14(5): 888–902. doi: 10.1261/rna.748408.
PMCID: PMC2327351

human; dendritic cells; cell differentiation; molecular biology; gene regulation

IgE-Antibody-Dependent Immunotherapy of Solid Tumors: Cytotoxic and Phagocytic Mechanisms of Eradication of Ovarian Cancer Cells

Sophia N. Karagiannis, Marguerite G. Bracher, James Hunt, Natalie McCloskey, Rebecca L. Beavil, Andrew J. Beavil, David J. Fear, Richard G. Thompson, Nicholas East, Frances Burke, Robert J. Moore, David D. Dombrowicz, Frances R. Balkwill, Hannah J. Gould

The Journal of Immunology, 2007, 179, 2832 -2843
Copyright © 2007 by The American Association of Immunologists, Inc.

IL-17 stimulates granulopoiesis in mice; use of an alternate novel gene therapy-derived method for in vivo evaluation of cytokines.

P. Schwarzenberger, V. La Russa, A. Miller, P. Ye, W. Huang, A. Zieske, S. Nelson, G.J. Bagby, D. Stoltz, R.L. Mynatt, M. Spriggs, J.K. Kolls

J Immunol, 1998 Dec 1;161(11):6383-9

Improved Clinical Scale Culture Conditions for Expansion of Cord Blood: Generation of Mature Neutrophil Cells.

I. Mcniece, E. J. Shpall, P. Kerzic, D. Kurbegov, S. Gross

The International Society for Hematotherapy and Graft Engineering (2000)

cord blood, Expansion, neutrophils transplant

In Vitro Production of Human Antigen Presenting Cells Issued From Bone Marrow of Patients With Cancer

V. Coulon, A. Ravaud, S. Huet, N. Gualde. 

Laboratoire d’Immunologie, Institut Bergonie, Centre Regional de Lutte Contre le Cancer, Bordeaux, Cedex, France. Universite Victor Segalen. Hematology and Cell Therapy. 1997. 39:237-244.

Antigen presenting cells; Human bone marrow; In vitro production

Inactivation of cytomegalovirus in platelet concentrates using helinx™ technology

Lily Lin

Seminars in Hematology
Volume 38, Supplement 11, October 2001, Pages 27-33 

Inducible Caspase 9 Suicide Gene to Improve the Safety of Allodepleted T Cells after Haploidentical Stem Cell Transplantation

Siok-Keen Tey, Gianpietro Dotti, Cliona M. Rooney, Helen E. Heslop, Malcolm K. Brenner

Biology of Blood and Marrow Transplantation, Volume 13, Issue 8, August 2007, Pages 913-924

Hematopoietic stem cell transplantation; Graft-versus-host disease; Gene therapy; T lymphocyte; Immunotherapy; Haploidentical

Inhibition of monocyte adhesion and fibrinogen adsorption on glow discharge plasma
deposited tetraethylene glycol dimethyl ether.

M. Shen, Y.V. Pan, M.S. Wagner, K.D. Hauch, D.G. Castner, B.D. Ratner, T.A. Horbett

J Biomater Sci Polym Ed. 2001;12(9):961-78

Innovative Packaging Of Frozen Dendritic Cell Vaccine

Nicolas Taquet

Large Animal Models for Stem and Progenitor Cell Analysis

Robert E. Donahue, Ken Kuramoto, Cynthia E. Dunbar

Current Protocols in Immunology
Copyright © 2007 by John Wiley and Sons, Inc.
DOI: 10.1002/0471142735.im22a01s69

stem cells; canine; feline; nonhuman primate; hematopoiesis; gene transfer; xenograft

Large Scale Ex Vivo Expansion and Activation of Human Natural Killer Cells for Autologous Therapy.

Miller, J.S., S. Klingsporn, J. Lund, E.H. Perry, C. Verfaillie, P. McGlave. 

Large scale production of human lymphokine activated killer cells for use in adoptive immunotherapy

Muul, L.M., E.P. Direcotr, C.L. Hyatt, S.A.Rosenberg. 

Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. Journal of Immunological Methods, 88. 1986. 265-275.

Human cytotoxic lymphocytes; adoptine immunotheropy; Recombinant interleukin 2; lymphokine activated killer; LAK

Large Volume Ex Vivo Expansion of CD34-Positive Hematopoietic Progenitor Cells for Transplantation

M.H. Purdy, C.J. Hogan, L. Hami, I. McNiece, W. Franklin, R.B. Jones, S.I. Bearman, R.J. Berenson, P.J. Cagnoni, S.Heimfeld, E.J. Shpall

JOURNAL OF HEMATOTHERAPY 4:515-525 (1995)

CD34; Hematopoietic ;myeloid; 

Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use 

Phil Lefebvre, Isaac Cohen 

Methods in Molecular Biology, vol. 215:
Cytokines and Colony Stimulating Factors: Methods and Protocols
Edited by: D. Körholz and W. Kiess © Humana Press Inc., Totowa, NJ 375-385

Large-Scale Expansion of the Natural Killer Cell Line NK-92 Under GMP Condions For Adoptive Cellular immunotherapy.

Y.K.Tam, K.'Doliqosa, J. Martinson, G. Maki, H.-G. Klingemann

Section of Bone Marrow Transplant and Cell Therapy, Rush Presbyterian-St. Luke's Medical Center, Chicago, IL, USA.

Large-Scale Isolation of CD34+ Cells Using the Amgen Cell Selection Device Results in High Levels of Purity and Recovery

I. Mcniece, R. Briddell, G. Stoney, B. Kern, K. Zilm, D. Recktenwald, S. Miltenyi

Journal Of Hematotherapy 6:5-11 (1997)

Linear polyamine copper chelator tetraethylenepentamine augments long-term ex vivo expansion of cord blood-derived CD34+ cells and increases their engraftment potential in NOD/SCID mice 

Toni Peled, Efrat Landau, Julie Mandel, Elina Glukhman, Noga R. Goudsmid, Arnon Nagler, Eitan Fibach

Experimental Hematology
Volume 32, Issue 6, June 2004, Pages 547-555 

Long Term Culture of EBV-Specific Ctls For Treatment Of Patients With Hodgkin's Disease In Cell Culture Bags

Helen Huls, Laura Aguilar, Barbara Savoldo, Helen Heslop, Cliona Roone

Baylor College of Medicine, Houston,TX (ND)

Long Term Engraftment in Breast Cancer Patients Who Received Ex Vivo Expanded PBPC

Ian McNiece, Julia Candia, Roy B. Jones, Scott I. Bearman, Pablo Cagnoni, Yago Nieto,
James Murphy, Elizabeth J. Shpall

Blood 96 (Supplement 1): 3628, 2000.

Breast cancer; PBPC

Loss of integrity of umbilical cord blood unit freezing bags: description and consequences

Bharat Thyagarajan, Michael Berger, Darin Sumstad, and David H. McKenna, Jr 

Transfusion, Volume 48 Issue 6, Pages 1138 - 1142 

Mechanical culture conditions effect gene expression: gravity-induced changes on the space shuttle

Hammond, T. G., E. Benes, K. C. O’Reilly, D. A. Wolf, R. M. Linnehan, J. H. Kaysen, P. L. Allen, and T. J. Goodwin

Physiol. Genomics 3: 163-173, 2000; 

Microgravity culture reduces apoptosis and increases the differentiation of a human colorectal carcinoma cell line

J. Milburn Jessue, Marylin Frantz, Enigul Sonmez-Alpan, Joseph Locker, Kimberly Skena, Heather Waller, Paul Battle, Alex Nachman, Mary Ellen Weber, Donald A. Thomas, Robert L. Curbeam, Jr., Tacey L. Baker, Thomas J. Goodwin

In Vitro Cell. Dev. Biol.--Animal 36:367-373, June 2000

microgravity; colon carcinoma; differentiation; apoptosis; CEA.

Microintegrating smooth muscle cells into a biodegradable, elastomeric fiber matrix 

John J. Stankusa, c, Jianjun Guanc, Kazuro Fujimotoc, William R. Wagner

Biomaterials, Volume 27, Issue 5, February 2006, Pages 735-744 

Bioreactor; Smooth muscle cell; Elastomer; Electrospinning; Polyurethane; Scaffold

Modulation of Cell Proliferation and Differentiation through Substrate-dependent Changes in Fibronectin Conformation

Andrés J. Garcia, Maria D. Vega, David Boettiger

Molecular Biology of the Cell, Vol. 10, 785-798, March 1999

Monoclonal Antibody Production in Serum-Free Medium

T. Giersch, S. Schapermeier

G.I.T. Laboralory Journal (1998) 113-114

hybridoma; teflon bag, pestdicides; 

Mouse microglial cell lines differing in constitutive and interferon-γ-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells 

William S. Walker, Janet Gatewood, Elvia Olivas, David Askew, Carin E. G. Havenith

Journal of Neuroimmunology
Volume 63, Issue 2, 31 December 1995, Pages 163-174 

Transgenic; Mouse; T cell receptor; Antigen presentation; CD4+ T cells; CD8 + T cells; Naive T cells; Memory T cells

Novel Methodological Approaches in Assessment and Enrichment of Stem Cell Population 

Nadja M. Bilko, Boris Fehse, Wolfram Ostertag, Carol Stocking, Axel R. Zander 

Stem Cells and their Potential for Clinical Application, 2008  

stem cell expansion; stem cell culture in vitro; diffusion chambers 

Novel replication-incompetent adenoviral B-group vectors: high vector stability and yield in PER.C6 cells

M. Havenqa, R. Voqels, D. Zuijdgeest, K. Radosevic, S. Mueller, M. Sieuwerts, F. Weichold, I. Damen, J. Kaspers, A. Lemckert, M. van Meerendonk, R. van der Vluqt, L. Holterman, D. Hone, Y. Skeiky, R. Mintardjo, G. Gillissen, D. Barouch, J. Sadoff, J. Goudsmit

Journal of General Virology (2006), 87, 2135-2148

Optimization of Conditions for Ex Vivo Expansion of CD34+ Cells From Patients with Stage IV Breast Cancer

Fred Shapiro, Tzy-Jyun Yao, George Raptis, Lilian Reich, Larry Norton, Malcolm A.S. Moore

Blood, Vol 84, No 10 (November 15), 1994: pp 3567-3574

Osteopontin Prevents Monocyte Recirculation and Apoptosis

Tricia H. Burdo, Malcolm R. Wood, Howard S. Fox

J Leukoc Biol. 2007 June ; 81(6): 1504–1511.

PEO-like plasma polymerized tetraglyme surface interactions with leukocytes and
proteins: in vitro and in vivo studies.

M. Shen, L. Martinson, M.S. Wagner, D.G. Castner, B.D. Ratner, T.A. Horbett

J Biomater Sci Polym Ed. 2002;13(4):367-90.

Peripheral Blood CD34 + Cells Differ From Bone Marrow CD34 + Cells in Thy-l Expression and Cell Cycle Status in Nonhuman Primates Mobilized or Not Mobilized With Granulocyte Colony-Stimulating Factor and/or Stem Cell Factor 

R.E. Donahue, M.R. Kirby, M.E. Metzger, B.A. Agricola, S.E. Sellers, H.M. Cullis

Blood, Vol 87, No 4 (February 15), 1996: pp 1644-1653

Phase I Study in Melanoma Patients of a Vaccine With Peptide-Pulsed Dendritic Cells Generated IN VITRO From CD34+ Hematopoietic Progenitor Cells.

Mackensen, A., B. Herbst, J-L. Chen, G. Kohler, C. Noppen, W. Herr, GC. Spagoli, V. Cerundolo, A. Lindemann. 

Dept of Hematology Oncology, Dept of Pathology, Freiburg University Medical Center, Freiburg, Germany.  

Physical and Thermal Properties of Blood Storage Bags: Implications for Shipping Frozen Products on Dry Ice

Thomas Reid

Walter Reed Army Institute of Research

Placebo-controlled study of intravenous magnesium supplementation during large-volume leukapheresis in healthy allogeneic donors

Salim Haddad, Susan F. Leitman, Robert A. Wesley, Stacey Cecco, Yu Ying Yau, Judith Starling, Nadja N. Rehak, Charles D. Bolan

Transfusion 2005;45:934-944.

Plasticizers - Under Scrutiny Again

J.R. Ellis

Medical Device & Diagnostic Industry, Circle Reader Service #57 (ND), 88-89

diethylhexyl phthalate (DEHP) 

Presence of IgE antibodies to bovine serum albumin in a patient developing anaphylaxis after vaccination with human peptide-pulsed dendritic cells

Andreas Mackensen, Ruth Dräger, Michael Schlesier, Roland Mertelsmann, Albrecht Lindemann

Cancer Immunol Immunother (2000) 49:152-156.

Dendriti

Purification of CD34+ Cells is Essential for Optimal Ex Vivo Expansion of Umbilical Cord Blood Cells

Robert A. Briddell, Brent P. Kern, Kerry L. Zilm, Gregory B. Stoney, Ian K. McNiece

Journal of Hematotherapy. 1997. 6:145-150.

Randomized placebo-controlled study of oral calcium carbonate administration in plateletpheresis: I. Associations with donor symptoms

Charles D. Bolan, Stacey A. Cecco, Yu Ying Yau, Robert A. Wesley, 

Transfusion 2003;43:1403-1413.

Randomized placebo-controlled study of oral calcium carbonate supplementation in plateletpheresis: II. Metabolic effects

Charles D. Bolan, Robert A. Wesley, Yu Ying Yau, Stacey A. Cecco, Judith Starling, Jaime M. Oblitas, Nadja N. Rehak, Susan F. Leitman

Transfusion 2003;43:1414-1422.

Rapid generation of broad T-cell immunity in humans after a single injection of mature dendritic cells.

Madhav V. Dhodapkar, R.M. Steinman, M. Sapp, H. Desai, C. Fossella, J. Krasovsky, S.M. Donahoe, P.R. Dunbar, V. Cerundolo, D.F. Nixon, N. Bhardwaj

Rapid Induction of Turnor-specific Type 1 T Helper Cells in Metastatic Melanoma Patients by Vaccination with Mature, Cryopreseeved, Peptide-loaded Monocyte-derived Dendritic Cells.

Beatrice Schuler-Thurner, 

J. Exp. Med. © The Rockefeller University Press s 0022-100712002/05/1279/10
Volume 195, Number 10, May 20,2002 1279-1288 

dendritic cells, vaccination, CD4+ T cells, 

Regeneration of Human Infarcted Heart Muscle by Intracoronary Autologous Bone Marrow Cell Transplantation in Chronic Coronary Artery Disease”

Bodo E. Strauer, Michael Brehm, Tobias Zeus, Thomas Bartsch, Christiana Schannwell, Christine Antke, R

Journal of the American College of Cardiology 2005;46:1651-1658.

Regulation of T Cell Immunity by Dendritic Cells

Antonio Lanzavecchla and Federica Sallusto

Cell, Vol. 106, 263-266, August 10, 2001

Relative Influence of Unfrozen Fraction and Salt Concentration on the Survival of Slowly Frozen Eight-Cell Mouse Embryos

U. Schneider, P. Mazur

Cryobiology. 1987. 24:17-41

Relevance and potential of natural killer cells in stem cell transplantation 

Hans G. Klingemann

Biology of Blood and Marrow Transplantation
Volume 6, Issue 2, March 2000, Pages 90-99 

Repair of Infarcted Myocardium by Autologous Intracoronary Mononuclear Bone Marrow Cell Transplantation in Humans

Bodo E. Strauer, Michael Brehm, Tobias Zeus, Matthias Köstering, Anna Hernandez, Rüdiger Sorg, Gesine Kögler, Peter Wernet

myocardial infarction, cell transplantation, intracoronary, angiogenesis, bone marrow, myogenesis

Retroviral Transduction and Expansion of Peripheral Blood Lymphocytes for the Treatment of Mucopolysaccharidosis Type II, Hunter’s Syndrome.

D.F. Stroncek, A. Hubel, R.A. Shankar, S.R. Burger, D. Pan, J. McCullough, G.B. Whitley           

Transfusion. 1999. 39:343-350.

Selective neuronal cell attachment to a covalently patterned monoamine on fluorinated ethylene propylene films

J.P. Ranieri, R. Bellamkonda, J. Jacob, T.G. Vargo, J.A. Gardella, P. Aebischer

J Biomed Mater Res. 1993 Jul;27(7):917-25

Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for Cartilage Tissue Repair.

M. Jin Kisiday, B. Kurz, H. Hung, C. Semino, Z. Zhang, A.J. Grodzinsky

PNAS, vol. 99, no. 15 ( 2002), 

three-dimensional cell culture ; biological scaffold ; regenerative medicine

Serum-Free Media Leads Growth in U.S. Cell Culture Markets

Nick Mariottini

Signaling Pathways Required for Macrophage Scavenger Receptor-mediated Phagocytosis: Analysis by Scanning Cytometry

Timothy H. Sulahian, Amy Imrich, Glen DeLoid, Aaron R. Winkler, Lester Kobzik

Respiratory Research 2008, 9:59 doi:10.1186/1465-9921-9-59

Simulated Microgravity Conditions Enhance Differentiation Of Cultured PC12 Cells Towards The Neuroendocrine Phenotype

Peter I. Lelkes, Daniel L. Galvan, G. Thomas Hayman, Thomas J. Goodwin, Dawn Y. Chatman, Sunu Cherian, Raul M. G. Garcia, Brian R. Unsworth

In VitroC ell. Dev. Biol.--Anima3l 4:316-325,A pril1 998
© 1998 Society for In Vitro Biology

PC12 cells; cell culture; PNMT; TH; differentiation; PCR; rotating wail vessel bioreactor; simulated microgravity.

Sources and Sequelae of Bacterial Contamination of Hematopoietic Stem Cell Components: Implications for the Safety of Hematotherapy and Graft Engineering

I.J. Webb, F.S. Coral, J.W. Andersen, A.D. Elias, R.W. Finberg, L.M. Nadler, J. Ritz, K.C. Anderson

Stromal Cells Derived from Spleen or Bone Marrow Support the Proliferation of Rat Natural Killer Cells in Long-Term Culture.

Amin Tjota, Thomas M. Rossi, Brian A. Naughton

Studies on the Ex Vivo Human Placental Transfer of HIV Infected Macrophages.

Bawdon, R.E., R.F. Larrson, and K. Magee. 

Superior Ex Vivo Cord Blood Expansion Following Co-culture with Bone Marrow-derived Mesenchymal Stem Cell

S.N. Robinson, J. Ng, T. Niu, H. Yang, J.D. McMannis, S. Karandish, I. Kaur, P. Fu, M. Del Angel, R. Messinger, F. Flagge, M. de Lima, W. Decker, D. Xing, R. Champlin, E.J. Shpall

Bone Marrow Transplant. 2006 February; 37(4): 359–366.
doi: 10.1038/sj.bmt.1705258.

cord blood, ex vivo expansion, mesenchymal stem cell, CD34+, CD133+, cobblestone area-forming cell assay

Teflon Bags for 5-Day Storage of Platelet Concentrates (PC) from
Cell Separator

V. Kretschmer, L. Cronemeyer, 

Infusionstherapie 1992;19:64 - 69

Plateletpheresis, Platelet storage, Teflon bags

The Host-Pathogen Interaction: New Themes from Dendritic Cell Biology

Maria Rescigno, Persephone Borrow

Cell, Vol. 106, 267-270, August 10, 2001

The In Vitro Growth of a Three-Dimensional Human Dermal Replacement Using a Single-Pass Perfusion System.

C.R. Halberstadt, Robert Hardin, K. Bezverkow, D. Snyder, L. Allen, L. Landeen. 

human dermal replacement; neonatal dermal fibroblasts; biosorbable polyglactin mesh; matrix proteins; bioreactor design; tissue engineering 

The Rationale and Use of Platelet Concentrates Irradiated with Ultraviolet-B Light

Derwood H. Pamphilon

Transfusion Medicine Reviews
Volume 13, Issue 4, October 1999, Pages 323-333 

The Use of Teflon Cell Culture Bags to Expand Functionally Active CD8+ Cytotoxic T Lymphocytes.

R.J. Garland, S.S. Kaneria, J.P. Hancock, C.G. Steward, A.W. Rowbottom. 

Transimmunization and The Evolution of Extracorporeal Photochemotherapy

Michael Girardi,Jeffrey Schechner, Earl Glusac, Carole Berger, Richard Edelson

Transfusionand Apheresis Science26 (2002) 181-190

Transimmunization; Extracorporealphotochemotherapy; Photopheresis; Dendritic cell; Antigen presentingcell; T-cell;
CutaneousT- celllymphoma;Immunotherapy;Anti-tumor

Transplantation of Adult and Pediatric Cancer Patients With Cord Blood Progenitors Expanded Ex Vivo

Elizabeth J. Shpall, Ralph Quinones, Roger Giller, Roy B. Jones, Scott I. Bearman, Pablo Cagnoni, Yago Nieto, Peter McSweeney, James Murphy, Chris Hogan, Alison Gocke, William Ward, Vicki Slat-Vasquez, Peggy Russell, Betsy Blunk, Marie Solzan, Ian McNiece

BMT Program, University of Colorado HSC, Denver, CO, USA

Cord blood transplantation; Pediatric transplantation

Transplantation of Enriched and Purged Peripheral Blood Progenitor Cells From a Single Apheresis Product in Patients With Non-Hodgkin’s Lymphoma.

R.S. Negrin, C.R. Kusnierz-Glaz, B.J. Still, J.R. Schriber, N.J. Chao, G.D. Long, C. Hoyle, W.W. Hu, S.J. Horning, B.W. Brown, K.G. Blume, Samuel Strober

Viability of Monocytes Grown in Teflon FEP Bags.

C. Hughes, P. Miller

Vitrification of Posterior Corneal Lamellae

Christian Meltendorf, Dirk K. Hinch, Friedrich Hoffmann

Cryobiology
Volume 44, Issue 2, April 2002, Pages 170-178 

Vitrification; Cryopreservation; Cornea; Cryopreservation vial; Berlin microkeratome; Posterior lamellar keratoplasty

Cyrobiophysical Characteristics of Genetically Modified Hematopoietic Progenitor Cells. 

Hubel, A., J. Norman, T.B. Darr. 

Cryobiology
Volume 38, Issue 2, March 1999, Pages 140-153 

water transport; hematopoietic stem cells; gene therapy

Transplantation of Ex vivo Expanded Cord Blood 

Elizabeth J. Shpall, Simon Robinson, Marcos de Lima, Krishna Komanduri, John D. McMannis, Laurence Cooper, Laura Worth, Chitra Hosing, Ian K. McNiece and Richard E. Champlin

Biology of Blood and Marrow Transplantation
Volume 13, Issue 11, November 2007, Page 1396 

Ex vivo expanded unselected peripheral blood: progenitor cells reduce posttransplantation neutropenia, thrombocytopenia, and anemia in patients with breast cancer

47. Paquette, R.L., S.T. Dergham, E. Karpf, H-J. Wang, D.J. Slamon, L. Souza, J.A. Glaspy. 

Blood. Oct 2000. Vol 96, No. 7.

How Cancer Vaccines Will Work

Kevin Bonsor

"How Cancer Vaccines Will Work." 

Growing Cholesterol-Dependent NSO Myeloma Cell Line in the Wave Bioreactor System: Overcoming Cholesterol-Polymer Interaction by Using Pretreated Polymer or Inert Fluorinated Ethylene Polymer. 

Kadarusman J, Bhatia R, McLaughlin J, Lin WR.