KryoSure® Cryopreservation
Freezing Bag Instructions (using the 2PF-0060 as an example):
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* A white cap is included inside a transparent 15x23cm overwrap. DO NOT DISCARD the overwrap, as it is used to contain the bag during freezing.
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1. Fill the bag through the sterile dockable tubing which enters the bag through the tubing containment pocket.
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2. Purge all the air out of the bag and then seal or clamp the tubing.
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3. Heat seal the sterile dockable tubing as close to the bag as possible. You may seal the tubing through the FEP plastic of the bag. Make additional segments as desired for sampling.
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4. Fold all tubing segments into the tubing containment pocket. Then seal the tubing containment pocket closed using a high temperature sealer such as the Accusealer of the Afc FEP4 (shown).
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5. After attaching any necessary labels, fold the label pocket alongside the bag. Insert the entire bag inside the transparent overwrap pouch.
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6. Heat seal the overwrap closed using two seals.
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